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The Nobel prize for the development of Green Fluorescent Protein (fusions) has opened up an entirely new way for biologists to study cellular processes. Unfortunately, this approach relies on the construction of fusion between GFP and the protein of interest. Moreover, there is no control of when and where this occurs. We have developed a new technology that overcomes both of these barriers, using near infra-red mediated release of labeled proteins from hollow gold nanoshells.

Related Publications

Shape Matters: Gold Nanoparticle Shape Impacts the Biological Activity of siRNA Delivery
Morgan E, Wupperfeld D, Morales DP, Reich NO. Bioconjugate Chem., 30(3) (2019), 853-860
https://doi.org/10.1021/acs.bioconjchem.9b00004

Affinity-Based Assembly of Peptides on Plasmonic Nanoparticles Delivered Intracellularly with Light Activated Control
Morales DP, Wonderly WR, Huang X, McAdams M, Chron AB, Reich NO. Bioconjugate Chem., 28(7) (2017), 1816-1820
https://doi.org/10.1021/acs.bioconjchem.7b00276

Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells
Huang X, Hu Q, Lai Y, Morales DP, Clegg DO, Reich NO. Adv. Mater., 28(48) (2016), 10732-10737
https://doi.org/10.1002/adma.201603318

Targeted Intracellular Delivery of Proteins with Spatial and Temporal Control
Morales DP, Braun GB, Pallaoro A, Chen R, Huang X, Zasadzinski JA, Reich NO. Mol. Pharm., 12(2) (2015), 600-609
https://doi.org/10.1021/mp500675p

Light-activated RNA interference in human embryonic stem cells
Huang X, Hu Q, Braun GB, Pallaoro A, Morales DP, Zasadzinski JA, Clegg DO, Reich NO. Biomaterials, 63 (2015), 70-79
https://doi.org/10.1016/j.biomaterials.2015.06.006

Modular Plasmonic Nanocarriers for Efficient and Targeted Delivery of Cancer-Therapeutic siRNA
Huang X, Pallaoro A, Braun GB, Morales DP, Ogunyankin MO, Zasadzinski JA, Reich NO. Nano Lett., 14(4) (2014), 2046-2051
https://doi.org/10.1021/nl500214e

Enzyme-Directed Positioning of Nanoparticles on Large DNA Templates
Braun GB, Dietchtierow M, Wilkinson S, Schmidt F, Hüben M, Weinhold E, Reich NO. Bioconjugate Chem., 19(2) (2008), 476-479
https://doi.org/10.1021/bc700275h

Specific and sensitive detection of nucleic acids and RNases using gold nanoparticle\[Dash]RNA\[Dash]fluorescent dye conjugates
Kim JH, Estabrook RA, Braun GB, Lee BR, Reich NO. Chem. Commun., (42) (2007), 4342
https://doi.org/10.1039/b710306a

Chemically Patterned Microspheres for Controlled Nanoparticle Assembly in the Construction of SERS Hot Spots
Braun GB, Pavel I, Morrill AR, Seferos DS, Bazan GC, Reich NO, Moskovits M. J. Am. Chem. Soc., 129(25) (2007), 7760-7761
https://doi.org/10.1021/ja072533e

Detection of Sequence-Specific Protein-DNA Interactions via Surface Enhanced Resonance Raman Scattering
Bonham AJ, Braun GB, Pavel I, Moskovits M, Reich NO. J. Am. Chem. Soc., 129(47) (2007), 14572-14573
https://doi.org/10.1021/ja0767837

Controlled Spacing of Cationic Gold Nanoparticles by Nanocrown RNA
Koyfman AY, Braun GB, Magonov S, Chworos A, Reich NO, Jaeger L. J. Am. Chem. Soc., 127(34) (2005), 11886-11887
https://doi.org/10.1021/ja051144m

Gold Nanoparticle Decoration of DNA on Silicon
Braun GB, Inagaki K, Estabrook RA, Wood DK, Levy E, Cleland AN, Strouse GF, Reich NO. Langmuir, 21(23) (2005), 10699-10701
https://doi.org/10.1021/la0515367